"I created all this sequencing data and have no idea how to run my microbiome analysis now. In the meantime my collaborators want me to look into associated bacteriophages and my mice need my attention this weekend. Where do I even start?"
"I'm spending more time wrangling raw sequencing files and hunting down missing metadata than actually learning new analysis methods. I need a standardized pipeline that just works across different microbiome datasets so I can focus on interpreting results, not babysitting data formats."
"My challenge is building a user-friendly platform for microbiome analysis that non-programmers can understand. I want everything to run reproducibly, but integrating all the niche tools (and keeping them updated) feels like an endless chase."
"I'm juggling grant deadlines, lab meetings, and mentoring PhD students. I need my team to generate publishable results without me having to micromanage every pipeline step so I can focus on steering the big-picture direction."